Invented by Roberto Falkenstein, Hubert Hertenberger, Petra Rueger, Tilman Schlothauer, Hoffmann La Roche Inc

Fc receptor-based affinity chromatography is a technique that has been used for the purification of monoclonal antibodies (mAbs) for several years. This technique is based on the interaction between the Fc region of an antibody and the Fc receptor of a protein A or protein G column. The Fc receptor-based affinity chromatography market is expected to grow significantly in the coming years due to the increasing demand for mAbs in the pharmaceutical industry. The global market for Fc receptor-based affinity chromatography is expected to grow at a CAGR of 8.5% from 2020 to 2025. The increasing demand for mAbs for the treatment of various diseases such as cancer, autoimmune disorders, and infectious diseases is driving the growth of this market. The use of mAbs has increased significantly in recent years due to their high specificity and low toxicity compared to traditional small molecule drugs. The Fc receptor-based affinity chromatography market is segmented based on product type, application, and end-user. The product type segment includes protein A, protein G, and others. Protein A is the most commonly used product type for Fc receptor-based affinity chromatography due to its high affinity for the Fc region of IgG antibodies. The application segment includes research, diagnostics, and therapeutics. The therapeutics segment is expected to grow at the highest CAGR during the forecast period due to the increasing demand for mAbs for the treatment of various diseases. The end-user segment includes pharmaceutical and biotechnology companies, academic and research institutes, and contract research organizations (CROs). The pharmaceutical and biotechnology companies segment is expected to hold the largest share of the market due to the increasing demand for mAbs in drug development and the commercialization of mAbs. North America is expected to hold the largest share of the Fc receptor-based affinity chromatography market due to the presence of a large number of pharmaceutical and biotechnology companies in the region. Europe is expected to be the second-largest market due to the increasing demand for mAbs for the treatment of various diseases. The Asia Pacific region is expected to grow at the highest CAGR during the forecast period due to the increasing investment in the biotechnology industry and the increasing demand for mAbs in the region. The key players operating in the Fc receptor-based affinity chromatography market include GE Healthcare, Thermo Fisher Scientific, Merck KGaA, Sartorius AG, Bio-Rad Laboratories, Pall Corporation, Novasep, Tosoh Corporation, Purolite Corporation, and Expedeon AG. These players are focusing on product development, collaborations, and partnerships to expand their market presence. In conclusion, the Fc receptor-based affinity chromatography market is expected to grow significantly in the coming years due to the increasing demand for mAbs in the pharmaceutical industry. The market is segmented based on product type, application, and end-user. North America is expected to hold the largest share of the market, while the Asia Pacific region is expected to grow at the highest CAGR during the forecast period. The key players operating in the market are focusing on product development, collaborations, and partnerships to expand their market presence.

The Hoffmann La Roche Inc invention works as follows

The use of an immobilized, non-covalent complex between a neonatal FcRn and beta-2-microglobulin as an affinity chromatography ligand is described here. This can be used, for instance, to determine the in-vivo half-life of an antibody, by measuring the ratio of retention times for an antibody and for a reference antibody.

Background for Fc receptor based affinity Chromatography

Specific Embodiment

EXAMPLES

Example 1

Preparation of FcRn affinity column

Example 2

Chromatography using the FcRn affinity column

Example 3

Correlation between Retention time on FcRm Column and In Vivo half life

Example 4

Purification of Human, Mouse and Cynomolgus fcRn

Example 5

Mouse & Cynomolgus Affinity Column Chromatographies:

Example 6

Generation of Antibody Fragments

Example 7

Correlation between Retention Time and Oxidation States on the FcRn Column

Example 8

The correlation of retention time on FcRn column to aggregate formation

Example 9

Pharmacokinetic study in human FcRn mice

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