Industrial Agricultural Biotech – Jay Short, Martin Keller, William Lafferty, BASF Enzymes LLC
Abstract for “High throughput or capillary based screening for a biological activity or biomolecule”
This method consists of enriching or screening a sample that contains polynucleotides derived from a mixed species of organisms. This method involves creating a DNA library using a variety of nucleic acids sequences from a mixed population and then separating the clones that contain a specific polynucleotide sequence. The analyzer detects a detectable molecular on a probe, or bioactive substrate. FACS, SQUID and MCS devices are all available to the analyzer. You can further process the enrich or separated library by either sequence based screening or activity-based screening. The enriched sequence can also be compared with a database to identify sequences that are homologous to clones in the library. This will allow you to obtain a nucleic acids profile for the mixed population.
Background for “High throughput or capillary based screening for a biological activity or biomolecule”
“EXAMPLE 1”
“DNA Isolation and Library Construction.”
“EXAMPLE 2”
“Construction a stable, large insert picoplankton Genomic DNA Library
“EXAMPLE 3”
“CsCl-Bisbenzimide Gradients”
“EXAMPLE 4”
“Bis-Benzimide Separation Genomic DNA”
“EXAMPLE 5”
“FACS/Biopanning”
“EXAMPLE 6”
“Large insert FACS Biopanning Protocol
“EXAMPLE 7”
“Biopanning Protocol”
“EXAMPLE 8”
“High Throughput Cultivation Marine Microbes From Sea Samples”
“EXAMPLE 9”
“Microextraction Procedure”
“EXAMPLE 11”
“Expression of Actinorhodin Pathway at S. venezuelae10712”
“EXAMPLE 12”
“Production Single Cells or Fragmented Mycelia.”
“EXAMPLE 13”
“REFERENCES”
“EXAMPLE 14”
“EXAMPLE 15”
“Identification of a novel bioactivity or biomolecule of interest by mass spectroscopic screening”
“RELATED REFERENCES”
“EXAMPLE 16”
“Plasmid DNA Transformation Protocol For Pseudomonas.”
“a. “a.
“b. Electroporation”
“EXAMPLE 17”
“Transformation of Yeast cells by Electoporation.”
“LITERATURE CITED”
Summary for “High throughput or capillary based screening for a biological activity or biomolecule”
“EXAMPLE 1”
“DNA Isolation and Library Construction.”
“EXAMPLE 2”
“Construction a stable, large insert picoplankton Genomic DNA Library
“EXAMPLE 3”
“CsCl-Bisbenzimide Gradients”
“EXAMPLE 4”
“Bis-Benzimide Separation Genomic DNA”
“EXAMPLE 5”
“FACS/Biopanning”
“EXAMPLE 6”
“Large insert FACS Biopanning Protocol
“EXAMPLE 7”
“Biopanning Protocol”
“EXAMPLE 8”
“High Throughput Cultivation Marine Microbes From Sea Samples”
“EXAMPLE 9”
“Microextraction Procedure”
“EXAMPLE 11”
“Expression of Actinorhodin Pathway at S. venezuelae10712”
“EXAMPLE 12”
“Production Single Cells or Fragmented Mycelia.”
“EXAMPLE 13”
“REFERENCES”
“EXAMPLE 14”
“EXAMPLE 15”
“Identification of a novel bioactivity or biomolecule of interest by mass spectroscopic screening”
“RELATED REFERENCES”
“EXAMPLE 16”
“Plasmid DNA Transformation Protocol For Pseudomonas.”
“a. “a.
“b. Electroporation”
“EXAMPLE 17”
“Transformation of Yeast cells by Electoporation.”
“LITERATURE CITED”
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